Pyrosequencing technology in a
24 well format
PyroMark™Q24 comes with a complete software package for the epigenetic variation study of CpG methylation, allele quantification and mutation analysis.
These studies are used for a wide range of clinical research, including the identification and tracking of changes involved in various forms of cancer. Studying these changes using Pyrosequencing technology, which is based on the principle of sequencing by synthesis, is an ideal choice for genetic analysis in clinical research. The output information is based on real sequence data providing integrated quality control and confidence in the quality of the results.
The 24 well format and the ability to run both mutation analysis and CpG assays on the same plate makes the PyroMark Q24 ideal for fewer samples, while providing the accuracy, flexibility and convenience of Pyrosequencing technology.
Results are shown in real time on the instrument display and as it takes only 15 minutes to run a typical SNP plate, multiple plates can be run each day.
The system is extremely easy to use. A control oligo delivered with the instrument ensures proper installation and a validation oligo is available to check the performance of the system.
PyroMark Q24 takes very little bench space, measuring only H420xW390xD525mm and it can be run at ambient temperatures between 15°C and 32°C.
PyroMark™Q24 System
PyroMark Q24 software can be installed on any computer that has the required specifications (Click here for specifications); it does not need to be connected to the PyroMark Q24 instrument. Information is transferred between the instrument and computer via a USB memory stick.
The system is delivered with two software analysis modes, CpG and AQ (Allele Quantification). Both of these modes can be used to analyze samples on the same plate, enabling different types of samples to be run at the same time. The software offers detailed report information that can be exported to html, excel, text and pdf formats.
AQ mode
Frequency calculations of variable positions in sequence context including:
• Single variable position AG/TC
• Multiple variable positions AG/TCAG/TCAG/T/AC
• Di- Tri- or Tetra-allelic mutations GA/C/G/TA
• High resolution of individual sites
• Quality assessment of individual sites and sequence context
Results from PyroMark Q24 software illustrating a K-RAS analysis of mutations in codon 12 and 13. The software automatically analyzes mutation levels and gives a quality scoring for all results, it also provides an informative plate overview, raw data (Pyrogram®), theoretical histogram, and provides report information that can be exported to html, excel, text and pdf formats.
CpG mode
Quantification of multiple consecutive CpG sites in sequence context;
• High resolution of individual sites
• Built-in control for bisulfite treatment
• Analyze methylation in the presence of SNPs
• Presents an overview of the deviation from specified methylation ranges
• Quality assessment of individual sites and sequence context
• Mean methylation for the whole assay can be shown for quick overview of results
Plate overview showing individual methylation frequencies and quality assessment for multiple, adjacent CpG sites. The above Pyrogram shows the raw data from well A5. Blue areas indicate variable CpG positions; yellow area indicates the built-in bisulfite treatment control.
PyroMark™Q24 Vacuum Prep Workstation -
Sample Preparation for the PyroMark Q24
In less than 15 minutes, PyroMark Q24 VPW (Vacuum Prep Workstation) prepares 24 samples in parallel from PCR product to single stranded template ready for sequencing.
- Reliable and simple preparation of DNA for Pyrosequencing
- Process up to 24 samples in parallel in less than 15 minutes
- Only 2 pipetting steps
- Vacuum Prep Tool designed for repeated use with re-usable filter probes
Product description
PyroMark Q24 VPW streamlines the sample preparation process, preparing 24 single-stranded DNA templates ready for primer annealing, in less than 15 minutes. The actual hands-on time is less than five minutes.
It accommodates six troughs for the different solutions necessary to process the samples.
The vacuum pump withstands mildly aggressive solutions and comes with a silencer.
Using the Vacuum Prep Workstation
Click here for instructions on how to use the Vacuum Prep Workstation
PyroMark™Q24 Validation Oligo
PyroMark™ Control Oligo
Two easy-to-use products are available for functional testing of PyroMark™Q24 System.
Both products consist of biotinylated oligonucleotides for an all-in-one verification of the
entire system, including sample preparation. The oligonucleotides have been designed to
promote formation of an internal stem-loop structure, thus eliminating the need for a separate
sequencing primer.
PyroMark Control Oligo and PyroMark Q24 Validation Oligo
The principle of Pyrosequencing® technology
Pyrosequencing technology offers an established genetic analysis method based on the principle
of sequencing by synthesis
- True sequence information
- Fully quantitative mutation analysis
- Easy to read and interpret
- Suitable for clinical research
Click here to read about Pyrosequencing chemistry, step by step
PyroMark Q24 specifications and ordering information
Click here for full specifications of PyroMark Q24 System
Click here for ordering information
Download
PyroMark™Q24 Product brochure Europe
PyroMark™Q24 Product brochure- US and Rest of World
PyroMark Control Oligo and PyroMark Q24 Validation Oligo